Tag
FLAG

Part:BBa_M10019:Design

Designed by: John Wang   Group: Berkeley BioE140L - S09   (2009-05-09)

{˂FLAG˃}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Later


Source

Wobble PCR Jw0019F / Jw0020R (45 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+ 910, L)
Product is pBca1256-K112602 {Flag}


Jw0019F Forward construction of Flag
cgataGAATTCatgAGATCTgactacaaggatgacgacg
Jw0020R Reverse construction of Flag
ccagtGGATCCcttgtcgtcgtcatccttgtagtc

References

Terpe K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. 60:523-33.